rabbit polyclonal anti inos antibody  (Proteintech)

Journal: Scientific Reports

Article Title: Barium titanate piezoelectric nanoparticles induce M1 polarization in mouse macrophages via ultrasound in vitro

doi: 10.1038/s41598-025-23364-6

Figure Lengend Snippet: ( A ) Fluorescence images of RAW264.7 cells under different conditions demonstrating bioelectrically-driven differentiation of M0 monocytes into M1 phenotype macrophages. Phase (Column 1), DAPI staining intensity (Column 2), and iNOS staining intensity (Column 3) are shown. Row 1 shows biochemical treatment with IFN-γ + LPS that drives M0 to M1. Row 2 shows positive iNOS intensity effect with pzNP and uS (+ NP, + uS). Rows 3, 4 and 5 represent controls with less/negligible iNOS staining intensity: with pzNPs and without uS (+ NP, –uS, Row 3), without pzNPs and with uS (–NP, + uS, Row 4), and without pzNPs or uS (-NP, -uS, Row 5). Note that the bioelectric combination of pzNP uptake and uS inducing iNOS expression in Row 2 is similar to biochemical treatment with IFN-γ + LPS in Row 1, both indicative of M0→M1 differentiation. ( B ) Average mean iNOS fluorescence intensity of cells within regions of interest from sets of n = 3 fluorescent images quantified using FIJI/ImageJ, for each NP/uS condition shown, plus that for IFN-γ + LPS. A p value > 0.1 (indicated as NS , for Not Significant) was determined via a t -test comparing the IFN-γ + LPS and (+ NP, + uS) data, while p < 0.01 (indicated as **) when comparing those with the three control data sets, as determined via ANOVA analysis. See text and Statistical Analysis for details.

Article Snippet: Then, a primary rabbit polyclonal antibody against mouse iNOS (Cell Signaling Technology, Danvers MA, no. 68186) or mouse arginase (Cell Signaling Technology, no. 93668), a marker for M1, was incubated overnight in PBS (1:500 dilution) at 4 °C.

Techniques: Fluorescence, Staining, Expressing, Control

Journal: Scientific Reports

Article Title: Barium titanate piezoelectric nanoparticles induce M1 polarization in mouse macrophages via ultrasound in vitro

doi: 10.1038/s41598-025-23364-6

Figure Lengend Snippet: Composite fluorescence images of a mixture of RAW264.7 macrophage cells exposed and not exposed to piezoelectric nanoparticle (pzNP) uptake, and then subjected to ultrasound (uS) stimulation . The channels displayed include phase contrast (panel A ), DAPI (blue, panel B ) for nuclear staining, iNOS expression (red, panel C ), and SA-FITC coating of pzNPs (green, panel D ), in cells subjected to pzNP uptake with uS stimulation. ( E ) A mixture of nanoparticle and non-nanoparticle loaded cells are detected. The results indicate that discrete subpopulations of cells exposed to pzNPs and uS can be identified. Cells that have taken up pzNPs but do not exhibit iNOS expression (green circle) can be differentiated from subpopulations that have taken up pzNPs and exhibit iNOS expression (white circle). There are subpopulations of cells that were not exposed to pzNPs and when subjected to ultrasound stimulation do not express iNOS expression (yellow circle).

Article Snippet: Then, a primary rabbit polyclonal antibody against mouse iNOS (Cell Signaling Technology, Danvers MA, no. 68186) or mouse arginase (Cell Signaling Technology, no. 93668), a marker for M1, was incubated overnight in PBS (1:500 dilution) at 4 °C.

Techniques: Fluorescence, Staining, Expressing